Fig. 5. Effects of a 12-week exposure of BEAS-2B cells to TPM from 3R4F reference cigarette smoke (7.5 µg/mL) and THS 2.2 aerosol (7.5 µg/mL, 37.5 µg/mL, and 150 µg/mL) on mitochondrial network morphology (A) and after 24h of starvation (B). Representative images of control (unexposed) cells and cells treated with DMSO, 7.5 µg/mL 3R4F reference cigarette smoke, and 7.5 µg/mL, 37.5 µg/mL, 150 µg/mL THS 2.2 aerosol TPM. Arrows indicate those cells in which the mitochondrial network is not completely connected. Red, mitochondria; blue, nuclei. Scale bar represents 10 μm. (C, D) The graphs show quantitative changes in the morphology of the mitochondrial network.